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Host Pathogen interaction in Brucellosis infection

Host-Pathogen Interactions

Research Question

  • To gain an improved understanding of how Brucella species enter, replicate and survive in and are released from infected cells

Research Gaps and Challenges

  • Brucella species infect macrophages which are an important contributor to the immune response so establishing how the bacterium interacts with macrophages is central to identifying the protective mechanisms and how the virus evades them.

Solution Routes

  • Brucella species and macrophage gene expression(transcriptome/RNA sequence data) in different in vivo environments (macrophages from naïve and immune hosts and in carrier animals)
  • Compare response where macrophages are infected with different Brucella species and strains, including s19, Rev 1 and RB51 looking at gene responses of the macrophage and the bacterium.
  • Comparison of the macrophage-bacteria response following clearance, in latency/carrier states and in active infections.
  • Identify disease stage biomarkers

Dependencies

  • The genome sequence of the various Brucella species, including s19, Rev 1 and RB51.

Projects

What activities are planned or underway?

EVOZOOM – Evolution and Zoonotic Epidemiology of Bovine Tuberculosis

Planned Completion date

Participating Country(s):

United KingdomIconUnited Kingdom

Investigating mechanisms of gallbladder persistence in Salmonella

Planned Completion date

Participating Country(s):

United KingdomIconUnited Kingdom