Coronaviruses roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic tests for coronaviruses
Download 202410 Draft Coronavirus Diagnostic Development_FinalA
Direct detection
Direct detection
Research Question
- Rapid detection of CoVs in different species and matrix
Research Gaps and Challenges
- Matrix specific effects
- Multispecies detection
- Cell-culture isolation of many CoVs is challenging
- Low viral load in field samples (lack of understanding of the disease pathogenesis = ? timing of sampling)
- Different genome constellations
- Mixed strains
- Unknown receptor specificity
Solution Routes
- Improvement of VI by new systems
- Improvement of systems for viral replication/growth
- Build broad range cell culture collections and organoids
- Modifying current available cells lines for being more sensitive to the different CoVs including dampened innate immunity and sensitive reporting system
Dependencies
- Basic knowledge on receptor usage
- Sharing of resources
- Training in genome manipulation technologies (CRISPR)
- Cell culture collections
State Of the Art
- Direct methods are available (e.g. virus isolation, electron microscopy and virus neutralization) but, being time consuming and laborious, are not envisaged for early detection, nor for surveillance
Projects
What activities are planned or underway?
Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy
Planned Completion date 31/01/2025
Source Countries:
Netherlands
Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation
Planned Completion date 28/02/2023
Source Countries:
Europe