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Coronaviruses roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic tests for coronaviruses

Download 202410 Draft Coronavirus Diagnostic Development_Final

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Direct detection

Direct detection

Research Question

  • Rapid detection of CoVs in different species and matrix

Research Gaps and Challenges

  • Matrix specific effects
  • Multispecies detection
  • Cell-culture isolation of many CoVs is challenging
  • Low viral load in field samples (lack of understanding of the disease pathogenesis = ? timing of sampling)
  • Different genome constellations
  • Mixed strains
  • Unknown receptor specificity

Solution Routes

  • Improvement of VI by new systems
  • Improvement of systems for viral replication/growth
  • Build broad range cell culture collections and organoids
  • Modifying current available cells lines for being more sensitive to the different CoVs including dampened innate immunity and sensitive reporting system

Dependencies

  • Basic knowledge on receptor usage
  • Sharing of resources
  • Training in genome manipulation technologies (CRISPR)
  • Cell culture collections

State Of the Art

  • Direct methods are available (e.g. virus isolation, electron microscopy and virus neutralization) but, being time consuming and laborious, are not envisaged for early detection, nor for surveillance