Coronaviruses roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic tests for coronaviruses
Download 202410 Draft Coronavirus Diagnostic Development_Final7
Host-pathogen interactions
Dependencies
Next steps
Host-pathogen interactions
Research Question
- Host pathogen interaction for developing a realistic diagnostic tool to address susceptible (7A) and Immune (7D)
Research Gaps and Challenges
- Susceptibility and pathogenesis of sarbecoviruses/merbecoviruses and embecoviruses etc is not completely understood.
- Signs due to immunopathology rather than virus specific pathology
- Distinguishing previous cross-reactive antibodies on ELISAs (e.g. Canine CoV inducing Ab which can be detected on Feline CoV test makes difficult to distinguish between exposure to different Coronavirus)
- Ct values on molecular tests do not correlate to infectious status
- Possible combination of assays including molecular and antibody tests to determine true status
Solution Routes
- Establishment of both in vitro and in vivo testing platforms, focusing on organoid and animal models
- Use of sgRNA (or replication intermediate) detection to supplement serological testing
- Use of virus isolation to demonstrate infectious virus is within the sample as can’t rely on qRT-PCR ct values (needs BSL3)
Dependencies
- Identification of entry receptors for specific CoV in different animal species
- Suitable testing platforms
- Organoids and tissue engineering to study coronavirus interactions in relevant hosts
Projects
What activities are planned or underway?
Differential susceptibility of SARS-CoV-2 in animals : Evidence of ACE2 host receptor distribution in companion animals, livestock and wildlife by immunohistochemical characterisation
Planned Completion date 26/07/2021
Participating Country(s):
Netherlands
Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation
Planned Completion date 28/02/2023
Participating Country(s):
Europe