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African Swine Fever roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic tests for ASF

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11

Direct detection/ identification

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Dependencies

Next steps

Improve direct identification of the ASF

Research Question

  • The improvement of direct identification of the ASF, especially direct ASFV detection via pen side testing

Research Gaps and Challenges

  • Further assessment of field-applicable PCR machines or other genome detection techniques

Solution Routes

  • Easy to use point-of-care molecular diagnostics

Dependencies

  • Improved DNA extraction
  • Direct PCR

State Of the Art

  • The most commonly used techniques for virus detection and identification are haemadsorption (HA), direct immunofluorescence (DIF), and since 2000, the molecular detection of ASF virus by PCR. While HA and DIF tests are not commercially available everywhere, several qPCRs are now on the market and licensed in several countries
  • qPCR is a very suitable gold standard for ASFV detection

Projects

What activities are planned or underway?

African Swine Fever virus inactivation by feed additives in vitro

Planned Completion date 01/01/2022

Source Countries:

NetherlandsIconNetherlands

The incursion risk of African swine fever for the Netherlands by human-mediated routes

Planned Completion date 01/01/2022

Source Countries:

NetherlandsIconNetherlands