Home African Swine Fever [Direct detection/ identification] Improve direct identification of the ASF
African Swine Fever roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic tests for ASF

Download ASF Diagnostic Roadmap

11

Direct detection/ identification

Dependencies

Next steps

Improve direct identification of the ASF

Research Question

  • The improvement of direct identification of the ASF, especially direct ASFV detection via pen side testing

Research Gaps and Challenges

  • Further assessment of field-applicable PCR machines or other genome detection techniques

Solution Routes

  • Easy to use point-of-care molecular diagnostics

Dependencies

  • Improved DNA extraction
  • Direct PCR

State Of the Art

  • The most commonly used techniques for virus detection and identification are haemadsorption (HA), direct immunofluorescence (DIF), and since 2000, the molecular detection of ASF virus by PCR. While HA and DIF tests are not commercially available everywhere, several qPCRs are now on the market and licensed in several countries
  • qPCR is a very suitable gold standard for ASFV detection

Projects

What activities are planned or underway?

Africa's Long Depression: The Growth and Debt Crises of 1975-2000

Planned Completion date 01/03/2027

Participating Country(s):

United KingdomIconUnited Kingdom