African Swine Fever roadmap:
Vaccines
Roadmap for the development of a candidate vaccine for ASF
Download ASF Vaccine RoadmapB
Replication
Replication
Research Question
- To generate knowledge on virus replication, as to allow the identification of the most suitable cell lines for virus replication
Research Gaps and Challenges
- Identify suitable cell lines for virus replication
- Produce an immortalised cell line for ASFV vaccine production
- Identify primary site of viral replication
- Identify alveolar macrophage genes that enable ASF viral growth to inform the development of a cell line for vaccine production
Solution Routes
- Generation of replication deficient viruses could contribute to the development of rationally attenuated vaccines
Dependencies
- Identify alveolar macrophage genes that enable ASF viral growth to inform the development of a cell line for vaccine production
State Of the Art
- ASFV replicates in the cytoplasm of the infected cell, primarily in discrete perinuclear assembly sites
- ASFV virions contain enzymatic activities that contribute to early events in, and activities critical for, viral replication in the cell cytoplasm, including RNA polymerase, nucleoside triphosphate phosphohydrolase, topoisomerase, mRNA capping, and protein kinase activity
- Implicated in macrophage host range are ASFV proteins involved in nucleotide and nucleic acid metabolism which may provide the deoxynucleotide pools favourable for efficient virus replication
- Deletion of the dUTPase (E165R gene) and thymidine kinase (K196R gene) genes from ASFV reduces its ability to replicate in macrophages
Projects
What activities are planned or underway?
African Swine Fever virus inactivation by feed additives in vitro
Planned Completion date 01/01/2022
Source Countries:
Netherlands
The incursion risk of African swine fever for the Netherlands by human-mediated routes
Planned Completion date 01/01/2022
Source Countries:
Netherlands