Coronaviruses roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic tests for coronaviruses
Download 202410 Draft Coronavirus Diagnostic Development_Final7
Host-pathogen interactions
Dependencies
Next steps
Host-pathogen interactions
Research Question
- Host pathogen interaction for developing a realistic diagnostic tool to address susceptible (7A) and Immune (7D)
Research Gaps and Challenges
- Susceptibility and pathogenesis of sarbecoviruses/merbecoviruses and embecoviruses etc is not completely understood.
- Signs due to immunopathology rather than virus specific pathology
- Distinguishing previous cross-reactive antibodies on ELISAs (e.g. Canine CoV inducing Ab which can be detected on Feline CoV test makes difficult to distinguish between exposure to different Coronavirus)
- Ct values on molecular tests do not correlate to infectious status
- Possible combination of assays including molecular and antibody tests to determine true status
Solution Routes
- Establishment of both in vitro and in vivo testing platforms, focusing on organoid and animal models
- Use of sgRNA (or replication intermediate) detection to supplement serological testing
- Use of virus isolation to demonstrate infectious virus is within the sample as can’t rely on qRT-PCR ct values (needs BSL3)
Dependencies
- Identification of entry receptors for specific CoV in different animal species
- Suitable testing platforms
- Organoids and tissue engineering to study coronavirus interactions in relevant hosts
Projects
What activities are planned or underway?
Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation
Planned Completion date 28/02/2023
Source Countries:
Europe
PARTNERSHIP: RAPID DETECTION OF INCURSIONS OF SARS-COV-2 AND NOVEL CORONAVIRUSES ON TEXAS MEAT AND DAIRY FARMS
Planned Completion date 31/08/2028
Source Countries:
United Kingdom