Coronaviruses roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic tests for coronaviruses
Download 202410 Draft Coronavirus Diagnostic Development_FinalA
AB response
Antibody response
Research Question
- Rapid and easy antibody detection (POC or LFT) to mitigate dependencies on virus neutralisation tests that take longer to perform
Research Gaps and Challenges
- What is the cross-reactivity amongst different CoV species – can this be supplemented with molecular testing?
- Potentially multiple CoV species circulating in animal populations
- Maternal immunity in herds/individuals
- Species-independent detection platforms
- Collection of antibody samples from postmortem tissues or other challenging matrices
- Lack of control reagents in diverse or wildlife species (i.e. secondary)
- Requirement for expertise and facilities for BSL3
Solution Routes
- Purification of antigens from different CoVs
- Study decline of humoral immunity of infected animals in different species
- Development of novel platforms for species-independent detection
- Development of surrogate testing to be performed at CL2/BSL2 in the absence of BSL3 – consideration that pseudotypes/VLPs may not fully recapitulate whole virus conditions
- Monoclonal antibody competition ELISA for cross-(host) species antibody detection
Dependencies
- Monoclonal Abs availability
- Rapid sharing of reagents
State Of the Art
- Multispecies N protein ELISA is available
- Several S specific ELISA are available too
- Multiplex surrogate virus neutralization test (sVNT)
Projects
What activities are planned or underway?
Differential susceptibility of SARS-CoV-2 in animals : Evidence of ACE2 host receptor distribution in companion animals, livestock and wildlife by immunohistochemical characterisation
Planned Completion date 26/07/2021
Participating Country(s):
Netherlands
Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation
Planned Completion date 28/02/2023
Participating Country(s):
Europe