Roadmap for the development of diagnostic test for influenza

Download Influenza-diagnostic-development-roadmap

AB response

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Next steps

Antibody response

Research Question

Rapid and easy antibody response detection

Research Gaps and Challenges

Rapid and easy detection of neutralization antibody, cross-reactivity antibody and antigenic characterization
Sensitvity of rapid assays
Inability to interpret antibodies in young animals – are they neutralizing? Are they maternally derived? Does their presence impact disease expression (VAERD or protection)?
Lack of true correlates of protection versus seroconversion
Understanding of the generation and decline of the humoral immunity of the infected and vaccinated birds

Solution Routes

ELISA and neutralizing antibody test development
In-field rapid test development and improvement
Antibody test platforms to evaluate cell mediated immune responses
Antibody test platforms to evaluate neutralizing antibodies.
Orchestrated development of monoclonal antibodies
Roadmap for the varying uses of antibody diagnostic approaches for veterinarians/responders based on purpose (DIVA vs. Early detection, etc.)

Dependencies

Monoclonal antibodies availability
Reference material collection
Field sample collection

State Of the Art

(2)Competitive ELISA based on NP protein is developed, and this can be used for the serum samples for many different avian species to see the seroconversion due to vaccination or natural infection