Roadmap for the development of diagnostic test for influenza

• Rapid and easy antibody response detection

• Rapid and easy detection of neutralization antibody, cross-reactivity antibody and antigenic characterization
• Sensitvity of rapid assays
• Inability to interpret antibodies in young animals – are they neutralizing? Are they maternally derived? Does their presence impact disease expression (VAERD or protection)?
• Lack of true correlates of protection versus seroconversion
• Understanding of the generation and decline of the humoral immunity of the infected and vaccinated birds

• ELISA and neutralizing antibody test development
• In-field rapid test development and improvement
• Antibody test platforms to evaluate cell mediated immune responses
• Antibody test platforms to evaluate neutralizing antibodies.
• Orchestrated development of monoclonal antibodies
• Roadmap for the varying uses of antibody diagnostic approaches for veterinarians/responders based on purpose (DIVA vs. Early detection, etc.)

• Monoclonal antibodies availability
• Reference material collection
• Field sample collection

(2)Competitive ELISA based on NP protein is developed, and this can be used for the serum samples for many different avian species to see the seroconversion due to vaccination or natural infection