African Swine Fever roadmap:
Vaccines
Roadmap for the development of a candidate vaccine for ASF
Download ASF Vaccine Roadmap13
Identity of virulence factors
Dependencies
Next steps
Identity of virulence factors
Research Question
- Identifying and removal of the factors contributing to pathological changes are essential for generating rationally attenuated vaccines
Research Gaps and Challenges
- Identify molecular differences in the pathogenesis process induced by virus with different degree of virulence
- Identify determinant of virulence
Solution Routes
- Swapping of suspected virulence genes between virulent and attenuated viruses
- Generation of a range of knock-out viruses and their use in experimental infections to establish the impact of the changes on virulence
Dependencies
- Improve understanding of virus macrophage interaction – viral and macrophage gene expression in different in vivo environments (macrophages from naïve and immune hosts)
- Perform comparative studies of the response to mild and highly virulent strains
- Identify primary site of viral replication
- Identify viral receptors and co-receptors
- Identify factors of persistence of infection (chronic infection)
State Of the Art
- Terminal genomic regions and Multigene Family (MGF) genes play a significant role in ASFV host range. Large deletion of six MGF360 genes and two MGF530 genes significantly reduce viral replication in macrophages and the virus pathogenesis in swine
- Deletion of either gene UK (DP96R) and 23-NL (DP71L or l14L), adjacently located in the genome although it does not affect viral growth in macrophages in vitro, does markedly attenuates the virus in swine
Projects
What activities are planned or underway?
African Swine Fever virus inactivation by feed additives in vitro
Planned Completion date 01/01/2022
Source Countries:
Netherlands
The incursion risk of African swine fever for the Netherlands by human-mediated routes
Planned Completion date 01/01/2022
Source Countries:
Netherlands