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Coronaviruses roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic tests for coronaviruses

Download 202410 Draft Coronavirus Diagnostic Development_Final

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Direct detection

Direct detection

Research Question

  • Rapid detection of CoVs in different species and matrix

Research Gaps and Challenges

  • Matrix specific effects
  • Multispecies detection
  • Cell-culture isolation of many CoVs is challenging
  • Low viral load in field samples (lack of understanding of the disease pathogenesis = ? timing of sampling)
  • Different genome constellations
  • Mixed strains
  • Unknown receptor specificity

Solution Routes

  • Improvement of VI by new systems
  • Improvement of systems for viral replication/growth
  • Build broad range cell culture collections and organoids
  • Modifying current available cells lines for being more sensitive to the different CoVs including dampened innate immunity and sensitive reporting system

Dependencies

  • Basic knowledge on receptor usage
  • Sharing of resources
  • Training in genome manipulation technologies (CRISPR)
  • Cell culture collections

State Of the Art

  • Direct methods are available (e.g. virus isolation, electron microscopy and virus neutralization) but, being time consuming and laborious, are not envisaged for early detection, nor for surveillance

Projects

What activities are planned or underway?