Influenza roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic test for influenza
Download Influenza-diagnostic-development-roadmapA
Direct detection
Direct detection
Research Question
- Rapid direct detection of influenza viruses and its variants
Research Gaps and Challenges
- Cost-effective and rapid direct detection of replication-competent influenza virus
- Matrix-specific effects
- Overcoming embryonated eggs as the main propagation vehicle retaining sample authenticity (re sample passage in cells/eggs)
- Host antibodies that neutralize virus
- Field based diagnostic for rapid early assessment with perhaps lab based confirmation/subtyping
- Genetic changes associated with different systems
- Low viral load in the field samples
- Mixed subtypes in field samples (coinfections)
Solution Routes
- Improvement of virus isolation and propagation by new in vitro cell culture techniques
- Improvement of systems for viral replication/growth
- Build cell culture collections
- Modify existing cell lines to be more sensitive by expressing or repressing genes
- Pen-side tests?
Dependencies
- Availability of SPF eggs in low resource settings
- Cell culture collections
- Crispr/Cas technology with avian cell culture systems
- Germ line manipulation tools in avian species
- Sharing of resources
- Early assessment linked with automatic alerts to advisors/central labs for additional tests
Projects
What activities are planned or underway?
Protect wildlife from livestock diseases.
Planned Completion date 07/10/2022
Participating Country(s):
Netherlands
Dynamics of sustainability in integrated agriculture : aquaculture systems in the Mekong Delta
Planned Completion date 21/10/2024
Participating Country(s):
Netherlands