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Influenza roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic test for influenza

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Direct detection

Direct detection

Research Question

  • Rapid direct detection of influenza viruses and its variants

Research Gaps and Challenges

  • Cost-effective and rapid direct detection of replication-competent influenza virus
  • Matrix-specific effects
  • Overcoming embryonated eggs as the main propagation vehicle retaining sample authenticity (re sample passage in cells/eggs)
  • Host antibodies that neutralize virus
  • Field based diagnostic for rapid early assessment with perhaps lab based confirmation/subtyping
  • Genetic changes associated with different systems
  • Low viral load in the field samples
  • Mixed subtypes in field samples (coinfections)

Solution Routes

  • Improvement of virus isolation and propagation by new in vitro cell culture techniques
  • Improvement of systems for viral replication/growth
  • Build cell culture collections
  • Modify existing cell lines to be more sensitive by expressing or repressing genes
  • Pen-side tests?

Dependencies

  • Availability of SPF eggs in low resource settings
  • Cell culture collections
  • Crispr/Cas technology with avian cell culture systems
  • Germ line manipulation tools in avian species
  • Sharing of resources
  • Early assessment linked with automatic alerts to advisors/central labs for additional tests

Projects

What activities are planned or underway?

Protect wildlife from livestock diseases.

Planned Completion date 07/10/2022

Participating Country(s):

NetherlandsIconNetherlands

Dynamics of sustainability in integrated agriculture : aquaculture systems in the Mekong Delta

Planned Completion date 21/10/2024

Participating Country(s):

NetherlandsIconNetherlands