African Swine Fever roadmap:
Vaccines
Roadmap for the development of a candidate vaccine for ASF
Download ASF Vaccine Roadmap3
Rationally attenuated candidates
Rationally attenuated candidates
Research Question
- Replicating ASFV are likely to give the most appropriate immune response but wild-type virus manipulates the host response. The aim is to reduce the virulence so that the vaccinated animal can mount a protective immune response. (The vaccine should allow the differentiation between infected and vaccinated animals)
Research Gaps and Challenges
- Lack of heterologous protection
- Possible link between immunogenicity and pathogenicity of the virus
- Ensure that vaccination prevents excretion of the organism – both the vaccine strain and wild type virus or any combination of the two that may have been generated
- Achieve long duration and fast onset of immunity
- Determine safety characteristics associated with experimental live attenuated
vaccines - Development of recombinant ASFV is still laborious
Solution Routes
- Identify suitable strains
- Create GM organisms lacking virulence and immunomodulatory factors
- Monitor the immune response following immunisation with the various candidates
- Develop improved systems to manipulate ASFV
- Perform challenge experiments with the various vaccine candidates, including challenge with other strains
- Identify more suitable cell lines for ASFV production
Dependencies
- Generate stable genetically modified attenuated organisms
- Identify virulence factors in ASFV
- Identify immunomodulators in ASFV
- Identify gene function ASFV
- Identify correlates of protection
- (Identify DIVA targets)
- Immune response: identify mechanism of immune evasion, and mechanism of protective immunity
- Better understand virus replication
- Develop suitable cell lines for production
State Of the Art
- Pigs immunised with live attenuated ASF viruses containing engineered deletions of specific ASFV virulence/host range genes could be protected to different levels when challenged with homologous parental virus
Projects
What activities are planned or underway?
African Swine Fever virus inactivation by feed additives in vitro
Planned Completion date 01/01/2022
Source Countries:
Netherlands
The incursion risk of African swine fever for the Netherlands by human-mediated routes
Planned Completion date 01/01/2022
Source Countries:
Netherlands