Influenza roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic test for influenza
Download Influenza-diagnostic-development-roadmap2
Validation/ proficiency
Validation
Research Question
- Validation of diagnostics tools
Research Gaps and Challenges
- Validation of existing diagnostics against novel isolates
- Lack of harmonized validation protocols
- Development of internal controls that are standardized and readily shared
- Validation of portable PCR
- Validation of serologic DIVA tests to understand levels of sampling to achieve goal to show freedom from disease
- Understand how NGS can be used to supplement/augment PCR testing
- Validation of use for minor species (9000 avian species!!) and age of animal (e.g. piglets and sows)
- The chances of independent and external validation are rare and its cost may be expensive
- Lack of reference material
Solution Routes
- Standardization of methods across labs – ring trials
- Co-ordinated lines of reference material production
- Orchestrated collections of field material
- Improve broad detection, sensitivity and specificity of diagnostic tests
- Validation of portable PCR
- Validation of serologic DIVA tests to understand levels of sampling to achieve goal to show freedom from disease
- Understand how NGS can be used to supplement/augment PCR testing
- Public/private partnerships – letting private companies have access to samples and share technologies with companies despite restrictions in sampling handling of material from HPAI/regulated diseases
Dependencies
- Reference materials
- Organizational structures of concerted validation lines.
- Availability of economic resources
Projects
What activities are planned or underway?
Protect wildlife from livestock diseases.
Planned Completion date 07/10/2022
Participating Country(s):
Netherlands
Impact of host age on viral and bacterial communities in a waterbird population.
Planned Completion date 01/11/2022
Participating Country(s):
Netherlands