African Swine Fever roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic tests for ASF
Download ASF Diagnostic Roadmap2
Validation
Dependencies
Next steps
Validation of reliable tests, including pens-side, being “fit for purpose” for surveillance, response, and recovery
Research Question
- Existing and novel diagnostics need to be validated in the field, according to different scenarios and different purposes (including
surveillance). ELISA tests for detection of antibodies need validation in alternative sample types. Available pen-side test needs to be
validated to enhance detection and improve surveillance also e.g. in wildlife in Africa
Research Gaps and Challenges
- Field validation should take into consideration global scenarios and the respective epidemiological contexts
- Validation in a wide range of alternative sample types
- Evaluation under experimental conditions of performance and overall accuracy of available ELISAs and PCR tests for surveillance
- Field validation of commercial confirmatory serological tests
- Standardization and validation of ELISA tests to detect antibodies against Ornithodoros tick saliva antigens in bitten animals
- Reduction of costs
- Easiness to use (from the point of view ISO 17025 accreditation)
Dependencies
- Reference materials
- Ring tests
- Training measures
State Of the Art
- Test systems for most questions available
- Validation studies are ongoing on several ASF diagnostics, including new ELISAs for antibody detection and pen-side ones (e.g. lateral flow devices, LFD)
Projects
What activities are planned or underway?
African Swine Fever virus inactivation by feed additives in vitro
Planned Completion date 01/01/2022
Source Countries:
Netherlands
The incursion risk of African swine fever for the Netherlands by human-mediated routes
Planned Completion date 01/01/2022
Source Countries:
Netherlands