Development of an innovative approach for early detection and on-site differential diagnosis of bacterial and fungal potato diseases (PoC-DiKa)

In Germany, potato farming faces threats from various bacterial and fungal pathogens, causing significant economic losses. To address this issue, the project aims to create an early warning system for detecting these diseases promptly. The goal is to prevent their spread and minimize yield losses. The project will explore two methods for early detection: analysing volatile substances emitted by diseased potatoes and utilizing a rapid point-of-care (PoC) test system directly in storage. An “electronic nose” system will be assessed for its suitability in detecting infected potato batches. Additionally, high-affinity monoclonal antibodies specific to these pathogens will be developed, and produced in an insect cell-based expression system to reduce costs. These antibodies will be integrated into a rapid test system, allowing for easy on-site identification by laypersons using a lateral flow assay. The methodology will involve using plant material from breeding companies to develop and validate the detection systems. Sensitivity tests will be conducted in specialized laboratories using molecular biological methods like qPCR and DNA barcoding with reference materials. Technical tests will further validate the systems during propagation on farms. The system’s modular design allows for future expansion to accommodate new pests or host plants. Ultimately, this project aims to safeguard potato crops against diseases, ensuring sustainable production practices.