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African Swine Fever roadmap:
Diagnostic Tests

Roadmap for the development of diagnostic tests for ASF

Download ASF Diagnostic Roadmap
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2

Validation
    "] --> 23188["

    1

    Diagnostics
      "] 23190["

      3

      Detection of host response
        "] --> 23189["

        2

        Validation
          "] 23192["

          4

          Expression system
            "] --> 23190["

            3

            Detection of host response
              "] 23193["

              5

              Stage-specific antigens
                "] --> 23192["

                4

                Expression system
                  "] 23194["

                  6

                  Immune response
                  • AResponse
                  • BCell-mediated immunity
                  "] --> 23193["

                  5

                  Stage-specific antigens
                    "] 23197["

                    7

                    Host-pathogen interactions
                    • AEntry
                    • BReplication
                    • CPersistence/ clearance
                    "] --> 23194["

                    6

                    Immune response
                    • AResponse
                    • BCell-mediated immunity
                    "] 23197["

                    7

                    Host-pathogen interactions
                    • AEntry
                    • BReplication
                    • CPersistence/ clearance
                    "] --> 23207["

                    12

                    Biomarker detection
                      "] 23201["

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                      Antigen detection
                        "] --> 23189["

                        2

                        Validation
                          "] 23201["

                          8

                          Antigen detection
                            "] --> 23194["

                            6

                            Immune response
                            • AResponse
                            • BCell-mediated immunity
                            "] 23202["

                            9

                            Genome characterization
                              "] --> 23201["

                              8

                              Antigen detection
                                "] 23202["

                                9

                                Genome characterization
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                                  2

                                  Validation
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                                    Genome detection
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                                      Genome characterization
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                                        Genome detection
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                                          2

                                          Validation
                                            "] 23204["

                                            11

                                            Direct detection/ identification
                                            • ASerotyping
                                            • BPhage typing
                                            "] --> 23189["

                                            2

                                            Validation
                                              "] 23207["

                                              12

                                              Biomarker detection
                                                "] --> 23189["

                                                2

                                                Validation
                                                  "] 23208["

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                                                  Organism isolation
                                                    "] --> 23197["

                                                    7

                                                    Host-pathogen interactions
                                                    • AEntry
                                                    • BReplication
                                                    • CPersistence/ clearance
                                                    "] 23208["

                                                    13

                                                    Organism isolation
                                                      "] --> 23201["

                                                      8

                                                      Antigen detection
                                                        "] 23208["

                                                        13

                                                        Organism isolation
                                                          "] --> 23202["

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                                                          Genome characterization
                                                            "] 23208["

                                                            13

                                                            Organism isolation
                                                              "] --> 23188["

                                                              1

                                                              Diagnostics
                                                                "] 23208["

                                                                13

                                                                Organism isolation
                                                                  "] --> 23204["

                                                                  11

                                                                  Direct detection/ identification
                                                                  • ASerotyping
                                                                  • BPhage typing
                                                                  "] 23209["

                                                                  14

                                                                  Sample collection/ transport/ preparation
                                                                    "] --> 23201["

                                                                    8

                                                                    Antigen detection
                                                                      "] 23209["

                                                                      14

                                                                      Sample collection/ transport/ preparation
                                                                        "] --> 23202["

                                                                        9

                                                                        Genome characterization
                                                                          "] 23209["

                                                                          14

                                                                          Sample collection/ transport/ preparation
                                                                            "] --> 23203["

                                                                            10

                                                                            Genome detection
                                                                              "] 23209["

                                                                              14

                                                                              Sample collection/ transport/ preparation
                                                                                "] --> 23204["

                                                                                11

                                                                                Direct detection/ identification
                                                                                • ASerotyping
                                                                                • BPhage typing
                                                                                "] 23209["

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                                                                                Sample collection/ transport/ preparation
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                                                                                  13

                                                                                  Organism isolation
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                                                                                    Lead Summary Challenges and gap analysis
                                                                                    1 Diagnostics State-of-the-art ASF diagnostics
                                                                                    2 Validation Validation of reliable tests, including pens-side, being “fit for purpose” for surveillance, response, and recovery
                                                                                    3 Detection of host response Develop diagnostic indirect tests for the detection of the disease
                                                                                    4 Expression system
                                                                                    5 Stage-specific antigens
                                                                                    6B Cell-mediated immunity
                                                                                    6A Response
                                                                                    6 Immune response Detection of immune response in ASFV infected animals
                                                                                    7C Persistence/ clearance
                                                                                    7B Replication
                                                                                    7A Entry
                                                                                    7 Host-pathogen interactions Investigate host-pathogen interaction in ASF infection
                                                                                    8 Antigen detection Identification of ASFV antigens for antigen capture ELISA and serological tests
                                                                                    9 Genome characterization ASFV genome characterization
                                                                                    10 Genome detection Improving sequencing of ASF genome
                                                                                    11B Phage typing
                                                                                    11A Serotyping
                                                                                    11 Direct detection/ identification Improve direct identification of the ASF
                                                                                    12 Biomarker detection Identify/develop cell lines that replace primary cultures for improved virus isolation techniques
                                                                                    13 Organism isolation Investigating alternative sample types for ASF diagnosis
                                                                                    14 Sample collection/ transport/ preparation